
%0 Journal Article
%A Batterham, TJ 
%A Shutt, DA 
%A Hart, NK 
%A Braden, AWH 
%A Tweeddale, HJ 
%T Metabolism of intraruminally administered [4-<sup>14</sup>C]formononetic and [4-<sup>14</sup>C]biochanin a in sheep
%J Australian Journal of Agricultural Research
%D 1971/02/01
%V 22
%N 1
%P 131-138
%K 
%R https://doi.org/10.1071/AR9710131
%U https://www.publish.csiro.au/paper/AR9710131 
%X Two ovariectomized ewes were given a single intraruminal dose of [4-14C]- labelled isoflavone and slaughtered 6 hr later. In the ewe given [4-14C]biochanin A, labelled genistein was detected in addition to labelled biochanin A in the blood plasma, urine, kidney, liver, bile, contents of the rumen, and those of the small intestine. p-Ethylphenol, which is almost certainly a metabolite of biochanin A, was present in the urine but not labelled, which indicates that it originates from ring B of the isoflavone molecule. In the ewe given [4-14C]formononetin, a similar distribution of the isoflavone and its demethylation product, daidzein, was found. In addition, labelled equol was found in the blood plasma and urine. Another labelled metabolite, identified as O-desmethyl angolensin (2,4-dihydroxyphenyl p-hydroxy-ß-methylbenzyl ketone), was also found in the urine. In the urine from both ewes, there were also labelled light petroleuminsoluble acidic compounds but they were not identified. 
