
TY  - JOUR
A1  - Hackman, RH 
A1  - Lazarus, Mahian 
T1  - Quantitative Analysis of Amino Acids Using Papre Chromatography
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1956
VL  - 9
IS  - 2
SP  - 281
EP  - 292
KW  - 
UR  - https://doi.org/10.1071/BI9560281 
N2  - A simple method for the quantitative analysis of amino [teids [n'eHent ill a protein hydrolysate, or in other mixtures of amino aeids, is deserihocl. The amino aeids are separated on one·dimensional paper chromatogmllls, fOUl" solvent syst;mns being used to resolvo 17 amino acids. The chromatogrums are treatm! with a suitable reagent; to detect the spots corresponding to each amino acid . .!Daeh chromatogram, {,ftcr being made somi·t,mllsparent with dimeth:vlphthalato, is scanned nutomnticnlly, with a densitometer, and the intensity of the light transmitted by the coloured spots is recorded on light-sensitive paper. A straight line relationship was found to hold, [or all amino acids, boi;wcell conccnh'ntioll and log per cent. transmission, Tho most useful range of amino aoid OOllcolltmtion was 2 5 mlYI although tho mothod is usable in tho rango 1-"10 mlVl. 'l'he method includes a nnmber of nOW tOclllli(juOS and tho HYOeage cooflicient of variation for a sillglo readillg for an an1ino n.eid is 5-7 pOl' COllt. 
ER  -

