
%0 Journal Article
%A Davern, CI 
%T The Isolation and Oharacterization of An Rna Bacteriophage
%J Australian Journal of Biological Sciences
%D 1964/09/01
%V 17
%N 3
%P 719-725
%K 
%R https://doi.org/10.1071/BI9640719
%U https://www.publish.csiro.au/paper/BI9640719 
%X An enrichment procedure for the isolation of RNA bacteriophage is described. The method involves the inoculation of sewage samples into cultures of Escherichia coli K-12 Hfr under conditions where DNA synthesis is restricted by the addition of 5-fiuorodeoxyuridine to the culture medium. Six phage isolates were made and all of them were shown to be male-specific. One of the male-specific phage was further characterized as an RNA phage, having very similar properties to RNA phage already isolated in other parts oftha world. This RNA phage has a buoyant density of 1·42 g/cm3 in caesium chloride. and has a sedimentation coefficient of 79'5 Sin O'Ol:M Tria-HOI buffer, pH 7· 4, at 20°0. 
