
TY  - JOUR
A1  - Russell, GR 
A1  - Smith, RM 
T1  - Reduction of Heliotrine by A Rumen Microorganism
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1968
VL  - 21
IS  - 6
SP  - 1277
EP  - 1290
KW  - 
UR  - https://doi.org/10.1071/BI9681277 
N2  - A small Gram-negative coccus, which brings about the reductive fission of the hepatotoxic pyrrolizidine alkaloids, has been isolated from the rumen contents of Australian Merino sheep. The organism was cultured in a partially defined medium, without rumen fluid, but containing yeast extract. Strictly anaerobic conditions and the presence of carbon dioxide were essential for growth. With limiting quantities of yeast extract, growth was greatly stimulated by any of several carbohydrates or moderately stimulated by the alkaloid heliotrine plus hydrogen. The products of the reductive cleavage of heliotrine were heliotric acid and 7()(-hydroxy-l-methylene- 8()(-pyrrolizidine. One mole of hydrogen reduced one mole of heliotrine to produce one mole each of the two products, accounting completely for the substrate consumed. Formate in stoichiometric quantities could replace molecular hydrogen as hydrogen donor in the reaction in which heliotrine acted as hydrogen acceptor. With [UCJformate as hydrogen donor 14CO. was evolved in stoichiometric amounts. The additional growth brought about by the degradation of heliotrine was proportional to the amount of heliotrine degraded, the organism appearing to derive energy from the reaction. Under strictly anaerobic conditions neither growth of the organism nor degradation of heliotrine was dependent on or stimulated by the presence of vitamin B12 in the medium. A method is described for the estimation of heliotrine. 
ER  -

