
TY  - JOUR
A1  - Mckenzie, HA 
A1  - Sawyer, WH 
T1  - On the Dissociation of Bovine ,b-Lactoglobulins A, B, and C Near Ph 7
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1972
VL  - 25
IS  - 5
SP  - 949
EP  - 962
KW  - 
UR  - https://doi.org/10.1071/BI9720949 
N2  - Sedimentation-equilibrium studies are made of the moleoular weights of bovine ,B.laotoglobulins A, B, and C at pH 7·5. The order of dissooiation of dimer to monomer is A> B ~ C. The dissooiation oonstant (Kd) for A and B is 0·6 X 10-4 and 0·08x 10-4 mole 1-1 at 20°C, respeotively. The oonditions ohosen for these measurements are based on sedimentation velooity studies in the pH range 6-9. There is no ohange in sedimentation velooity behaviour following different times of standing at 20°C for pH < 7·5. The sedimentation patterns exhibit a single peak with some trailing on the solvent side. At low oonoentrations the plot of weight average sedimentation coefficient (,~) versus concentration (0) is in aocord with that of a rapidly dissociating system of monomer-dimer type. There are time-dependent aggregations above pH 8. Effects of changes in ionic strength and addition of methanol are considered. 
ER  -

