
%0 Journal Article
%A Skyring, GW 
%A Jones, HE 
%T Variations in the Spectrum of Desulfoviridin From Desulfovibrio gigas
%J Australian Journal of Biological Sciences
%D 1976/09/01
%V 29
%N 4
%P 291-300
%K 
%R https://doi.org/10.1071/BI9760291
%U https://www.publish.csiro.au/paper/BI9760291 
%X Desulfoviridin preparations from D. gigas showed variations in the position < of the absorption maximum (the p-peak) in the 580-nm region of the spectrum. On treatment with Na2S204 a preparation with a p-peak at 585 nm was affected rapidly, the 585-nm peak shifting to the 596-nm region; this was partially reversed by K3Fe(CN)6. Treatment of the original preparation with K3Fe(CN)6 resulted in a shift of the p-peak to 582-583 nm. Desulfoviridins with p-peaks from 580 to 583 nm were not rapidly affected by Na2S204. The spectrum of the chromophore of desulfoviridin was also affected by Na2S204 with the peak at 587 nm shifting to 597 nm; this effect was completely reversed by oxygen. There was no evidence to <show that spectral variations in desulfoviridin preparations were due to the loss or acquisition of metal ions during growth or to the selection of mutants containing spectrally different desulfoviridins. It is suggested that during biosynthesis or purification, desulfoviridin may be structurally modified, allowing partial detachment of the chromophore, thus causjng a change towards the spectral properites of the detached chromophore. 
