
%0 Journal Article
%A P Coghlan, John 
%A SK Fan, James 
%A A Scoggins, Bruce 
%A A Shulkes, Arthur 
%T Measurement of Extracellular Fluid Volume and Blood Volume in Sheep
%J Australian Journal of Biological Sciences
%D 1977/02/01
%V 30
%N 2
%P 71-84
%K 
%R https://doi.org/10.1071/BI9770071
%U https://www.publish.csiro.au/paper/BI9770071 
%X Methods are described for the simultaneous measurement of extracellular fluid volume (ECFV) and plasma volume (PV) in sheep using dilution of 82Br (as sodium bromide) and l3lI-labelled ovine gamma globulin. Following injection of 82Br (100 }lCi), equilibrium in blood was reached after 3 h at which time only 4 % of the injected dose was in rumen water. The ECFV was measured as the mean Of the 2- and 3-h bromide space after correction for the relative water content of plasma, the Gibbs-Donnan factor and the loss of 82Br into red blood cells. l 3lI-labelled ovine gamma globulin (20 }lCi) was injected after the 3-h 82Br space was obtained and blood samples were taken at 10, 20, 30 and 40 min. 
