
TY  - JOUR
A1  - P Coghlan, John 
A1  - SK Fan, James 
A1  - A Scoggins, Bruce 
A1  - A Shulkes, Arthur 
T1  - Measurement of Extracellular Fluid Volume and Blood Volume in Sheep
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1977
VL  - 30
IS  - 2
SP  - 71
EP  - 84
KW  - 
UR  - https://doi.org/10.1071/BI9770071 
N2  - Methods are described for the simultaneous measurement of extracellular fluid volume (ECFV) and plasma volume (PV) in sheep using dilution of 82Br (as sodium bromide) and l3lI-labelled ovine gamma globulin. Following injection of 82Br (100 }lCi), equilibrium in blood was reached after 3 h at which time only 4 % of the injected dose was in rumen water. The ECFV was measured as the mean Of the 2- and 3-h bromide space after correction for the relative water content of plasma, the Gibbs-Donnan factor and the loss of 82Br into red blood cells. l 3lI-labelled ovine gamma globulin (20 }lCi) was injected after the 3-h 82Br space was obtained and blood samples were taken at 10, 20, 30 and 40 min. 
ER  -

