
TY  - JOUR
A1  - Macmillan, KL 
A1  - Hart, NL 
T1  - Effects of Low Concentrations of Cetyltrimethylammonium Bromide on Diluted Bovine Spermatozoa
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1977
VL  - 30
IS  - 4
SP  - 297
EP  - 302
KW  - 
UR  - https://doi.org/10.1071/BI9770297 
N2  - Cetyltrimethylammonium bromide (CTAB) at a concentration of 10 mg/l in a citrate buffer partially protected sperm against the effects of cold shock treatment. CT AB concentrations of 10 and 20 mg/l also increased in vitro sperm livability when semen was diluted in Caprogen and incubated at 37°C (50 h v. 60 and 57 h). Concentrations of CTAB exceeding 50 mg/l reduced in vitro sperm livability. The addition of catalase (4· 5 mgfl) to Caprogen produced greater increases in in vitro sperm livability (50 h v. 110 h), but did not eliminate the toxic effect of the higher CTAB concentrations. The addition of CTAB (10 mg/l) to the Caprogen-catalase diluent significantly increased the non-return rate of the diluted semen in only one of four large-scale field trials and did not alter non-return rates with deep-frozen semen. 
ER  -

