
%0 Journal Article
%A Elleman, TC 
%A Falconer, IR 
%A Jackson, ARB 
%A Runnegar, MT 
%T Isolation, Characterization and Pathology of the Toxin From a Microcystis Aeruginosa (= Anacystis Cyanea) Bloom
%J Australian Journal of Biological Sciences
%D 1978/06/01
%V 31
%N 3
%P 209-218
%K 
%R https://doi.org/10.1071/BI9780209
%U https://www.publish.csiro.au/paper/BI9780209 
%X The nature of the toxicity of a bloom of blue-green alga, M. aeruginosa (= Anacystis cyanea), that occurred in a man-made lake was investigated. Crude algal bloom extracts were toxic to laboratory mice when injected intraperitoneaIIy. The lethal dose (LDlOO) of these extracts was 15-30 mg of lyophilized algal bloom per kilogram body weight. The toxin was purified by a procedure that included ammonium sulphate fractionation, solvent extraction, acid precipitation, Sephadex G25 and DEAE-Sephadex chromatography, and high-voltage electrophoresis at pH 6·5. The preparation gave a single spot on high-voltage electrophoresis at pH 9·0, had no free amino group, and was characterized by a simple amino acid composition of equimolar quantities of L-methionine, L-tyrosine, D-alanine, D-glutamic acid, erythro fi-methyl aspartic acid and methylamine. 
