
%0 Journal Article
%A M Harlow, Gaye 
%A Quinn, P 
%T Isolation of Inner Cell Masses From Mouse Blastocysts by Immunosurgery or Exposure to the Calcium Ionophore A23187
%J Australian Journal of Biological Sciences
%D 1979/10/01
%V 32
%N 5
%P 483-492
%K 
%R https://doi.org/10.1071/BI9790483
%U https://www.publish.csiro.au/paper/BI9790483 
%X Two techniques have been evaluated for their use in routinely isolating inner cell masses from mouse blastocysts by destroying the trophectoderm. The most efficient method of immunosurgery was a 15-min incubation in a 1 : 50 dilution of rabbit anti-mouse spleen antiserum followed by a 30-60-min incubation in guinea pig complement (1: 10). Alternatively, inner cell masses were isolated by incubating blastocysts in 1O-5M calcium ionophore A23187 in medium devoid of calcium and magnesium ions. 
