
TY  - JOUR
A1  - M Harlow, Gaye 
A1  - Quinn, P 
T1  - Isolation of Inner Cell Masses From Mouse Blastocysts by Immunosurgery or Exposure to the Calcium Ionophore A23187
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1979
VL  - 32
IS  - 5
SP  - 483
EP  - 492
KW  - 
UR  - https://doi.org/10.1071/BI9790483 
N2  - Two techniques have been evaluated for their use in routinely isolating inner cell masses from mouse blastocysts by destroying the trophectoderm. The most efficient method of immunosurgery was a 15-min incubation in a 1 : 50 dilution of rabbit anti-mouse spleen antiserum followed by a 30-60-min incubation in guinea pig complement (1: 10). Alternatively, inner cell masses were isolated by incubating blastocysts in 1O-5M calcium ionophore A23187 in medium devoid of calcium and magnesium ions. 
ER  -

