
%0 Journal Article
%A Potter, WL 
%A Upton, PC 
%A Dunn, BL 
%T Morphological Changes as Observed by Light Microscopy of the Acrosome of Boar Spermatozoa Subjected to Deep Freezing
%J Australian Journal of Biological Sciences
%D 1979/12/01
%V 32
%N 6
%P 575-580
%K 
%R https://doi.org/10.1071/BI9790575
%U https://www.publish.csiro.au/paper/BI9790575 
%X The effects of chilling (5°C) and deep freezing in liquid nitrogen (-196°C) on the acrosomal morphology of diluted boar spermatozoa were evaluated by light microscopy using Giemsa stain. A large reduction in numbers of spermatozoa with normal acrosomes was recorded as the temperature fell from 30 to 5°C but the greatest damage occurred during the freeze-thaw cycle. 
