
TY  - JOUR
A1  - Potter, WL 
A1  - Upton, PC 
A1  - Dunn, BL 
T1  - Morphological Changes as Observed by Light Microscopy of the Acrosome of Boar Spermatozoa Subjected to Deep Freezing
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1979
VL  - 32
IS  - 6
SP  - 575
EP  - 580
KW  - 
UR  - https://doi.org/10.1071/BI9790575 
N2  - The effects of chilling (5°C) and deep freezing in liquid nitrogen (-196°C) on the acrosomal morphology of diluted boar spermatozoa were evaluated by light microscopy using Giemsa stain. A large reduction in numbers of spermatozoa with normal acrosomes was recorded as the temperature fell from 30 to 5°C but the greatest damage occurred during the freeze-thaw cycle. 
ER  -

