
%0 Journal Article
%A GR Hurrell, John 
%A M Malouf, PauI 
%A Conway, Richard 
%T A New Insulin Derivative for the Preparation of Insulin-Enzyme Conjugates: An Application in the Enzyme Immunoassay of Insulin
%J Australian Journal of Biological Sciences
%D 1980/12/01
%V 33
%N 6
%P 633-642
%K 
%R https://doi.org/10.1071/BI9800633
%U https://www.publish.csiro.au/paper/BI9800633 
%X A new insulin derivative, mercaptobutyrimidyl pyridine disulfide insulin, was prepared under conditions which directed the reaction preferentially through the terminal (X-amino group of the A-chain. The purified monosubstituted insulin exhibited only a marginal decrease in antigenicity as measured by radioimmunoassay; there was, however, a significant reduction in biological activity in the mouse convulsion assay. Conjugation of the insulin derivative to human placental alkaline phosphatase was carried out via a thiol interchange reaction and the resulting conjugate was active in a double-antibody, solid-phase enzyme immunoassay for insulin. 
