
%0 Journal Article
%A Mettler, L 
%A Skrabei, H 
%A Czuppon, AB 
%T Detection of Human Spermatozoal Peptides After Conjugation to 125J-Labelled Human Serum Albumin
%J Australian Journal of Biological Sciences
%D 1981/12/01
%V 34
%N 6
%P 587-592
%K 
%R https://doi.org/10.1071/BI9810587
%U https://www.publish.csiro.au/paper/BI9810587 
%X Human spermatozoal peptides, liberated during autolysis of the cells, were fractionated by gelfiltration chromatography and thin-layer chromatography. After conjugation to 125I-labeIIed human serum albumin, all fractions were assayed with rabbit antihuman spermatozoa antiserum. The reactions indicated the presence of a number of antigenic peptides in the spermatozoal autolysate and one of the fractions revealed a significantly higher antibody binding capacity than all other fractions assayed (P < 0·05). Release of immunogenic peptides during autolysis of the spermatozoal membrane could be important in the aetiology of spermatozoal iso- and autoantibodies. 
