
TY  - JOUR
A1  - S Mohapatra, S 
A1  - GresshoffA, PM 
T1  - Sensitivity to Oxygen of Nitrogenase Activity in Rhizobium Strain ANU289 of the Non-legume Parasponia (Ulmaceae)
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1984
VL  - 37
IS  - 2
SP  - 31
EP  - 36
KW  - 
UR  - https://doi.org/10.1071/BI9840031 
N2  - Nitrogenase activity can be detected in Rhizobium strain ANU289 of Parasponia in shaken liquid cultures. A combination of consistently low oxygen tension and appropriate cell density was found to be essential for rapid initiation and high specific rates (in the range of 50-60 nmoles of ethylene per milligram protein per hour). In the presence of succinate as carbon source and an oxygen concentration of 0·25% (v/v) in the gas phase, nitrogenase activity developed after incubation for 24 h. The requirement for an oxygen concentration in the range of O· 1 to 0·35% for derepression indicated a lack of any inherent tolerance to higher oxygen levels and thus suggests that plant-derived mechanisms are responsible for protection by oxygen in the nodule. 
ER  -

