
%0 Journal Article
%A Annison, Geoffrey 
%A Couperwhite, lain 
%T Composition of Alginate Synthesized during the Growth Cycle of Pseudomonas aeruginosa
%J Australian Journal of Biological Sciences
%D 1987/12/01
%V 40
%N 4
%P 435-442
%K 
%R https://doi.org/10.1071/BI9870435
%U https://www.publish.csiro.au/paper/BI9870435 
%X A mucoid P. aeruginosa isolated from the sputum of a cystic fibrosis patient was grown in batch culture on a complex medium. During the growth cycle the amount of alginate produced was estimated and its composition was determined by proton magnetic resonance (IH-n.m.r) spectroscopy. Exopolysaccharide production occurred mainly during the exponential phase of growth. The alginate samples isolated varied little in composition and were characterized by being highly acetylated, high mannuronate (0.83 - 0.93 mole fraction) polymers. Guluronate was present only within heteropolymeric regions of the polysaccharides which all displayed a complete absence of polyguluronate. Ca2 + ion supplementation of the medium was not observed to increase the levels of guluronate in the algi nates produced. 
