
%0 Journal Article
%A O'Keefe, IH 
%A Sharry, LF 
%A Panaretto, BA 
%T The Fate of Tritiated rm-Epidermal Growth Factor in the Sheep: Validation of the Labelling Procedure and Rate of Tissue Clearance
%J Australian Journal of Biological Sciences
%D 1988/12/01
%V 41
%N 4
%P 539-552
%K 
%R https://doi.org/10.1071/BI9880539
%U https://www.publish.csiro.au/paper/BI9880539 
%X Plasmid-derived recombinant mouse epidermal growth factor, rm-EGF, was purified by ion pair reversed phase high performance liquid chromatography. The product peak (termed rm-a-EGF) was characterized by physicochemical techniques including fast atom bombardment mass spectrometry, high field proton magnetic resonance and amino acid sequencing (amino acid arrangement and composition). The rm-a-EGF was tritiated, labile tritium removed by lyophilization: and the product purified and characterized as for the parent compound to yield a compound identical to rm-a-EGF except for the isotopic hydrogen substitution. Label stability was validated by lyophilization of samples, especially urine. 
