
TY  - JOUR
A1  - O'Keefe, IH 
A1  - Sharry, LF 
A1  - Panaretto, BA 
T1  - The Fate of Tritiated rm-Epidermal Growth Factor in the Sheep: Validation of the Labelling Procedure and Rate of Tissue Clearance
JF  - Australian Journal of Biological Sciences
JO  - Aust. Jnl. Of Bio. Sci.
Y1  - 1988
VL  - 41
IS  - 4
SP  - 539
EP  - 552
KW  - 
UR  - https://doi.org/10.1071/BI9880539 
N2  - Plasmid-derived recombinant mouse epidermal growth factor, rm-EGF, was purified by ion pair reversed phase high performance liquid chromatography. The product peak (termed rm-a-EGF) was characterized by physicochemical techniques including fast atom bombardment mass spectrometry, high field proton magnetic resonance and amino acid sequencing (amino acid arrangement and composition). The rm-a-EGF was tritiated, labile tritium removed by lyophilization: and the product purified and characterized as for the parent compound to yield a compound identical to rm-a-EGF except for the isotopic hydrogen substitution. Label stability was validated by lyophilization of samples, especially urine. 
ER  -

