
%0 Journal Article
%A Khanam, Nurussaba 
%A Khoo, Cheang 
%A Khan, Abdul G. 
%T Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of <emph type="2">Duboisia myoporoides</emph>
%J Australian Journal of Botany
%D 2001/08/02
%V 49
%N 4
%P 443-450
%K 
%R https://doi.org/10.1071/BT00064
%U https://www.publish.csiro.au/paper/BT00064 
%X In non-rooted shoots regenerated from calluses induced with nine different cyokinin&#8211;auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokinin&#8211;auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 &#181;M + naphthaleneacetic acid (NAA) 1 &#181;M and BA 10 &#181;M + indole-3-butyric acid (IBA) 0.1 &#181;M. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 &#215; 10 &#8211;4 and 6.5 &#215; 10 &#8211;4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokinin&#8211;auxin combinations. No tropane alkaloids were detected in these shoots. 
