
TY  - JOUR
A1  - Khanam, Nurussaba 
A1  - Khoo, Cheang 
A1  - Khan, Abdul G. 
T1  - Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of <emph type="2">Duboisia myoporoides</emph>
JF  - Australian Journal of Botany
JO  - Aust. J. Bot.
Y1  - 2001
VL  - 49
IS  - 4
SP  - 443
EP  - 450
KW  - 
UR  - https://doi.org/10.1071/BT00064 
N2  - In non-rooted shoots regenerated from calluses induced with nine different cyokinin&#8211;auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokinin&#8211;auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 &#181;M + naphthaleneacetic acid (NAA) 1 &#181;M and BA 10 &#181;M + indole-3-butyric acid (IBA) 0.1 &#181;M. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 &#215; 10 &#8211;4 and 6.5 &#215; 10 &#8211;4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokinin&#8211;auxin combinations. No tropane alkaloids were detected in these shoots. 
ER  -

