
TY  - JOUR
A1  - Astarini, I. A. 
A1  - Yan, G. 
A1  - Plummer, J. A. 
T1  - Interspecific Hybridisation of Boronias
JF  - Australian Journal of Botany
JO  - Aust. J. Bot.
Y1  - 1999
VL  - 47
IS  - 6
SP  - 851
EP  - 864
KW  - 
UR  - https://doi.org/10.1071/BT98017 
N2  - Interspecific hybridisation within the genus Boronia was attempted by using species with a range of chromosome numbers. Crosses were possible between Western Australian species including those with B. heterophylla as the female parent and pollen from B. molloyae (n = 8), B. megastigma (n = 7), B. purdienana (n = 9) and B. ramosa (n = 18). Cytological studies revealed that B. heterophylla genotypes had different chromosome numbers; &#8216;Red&#8217; had 2n = 15, whereas &#8216;Moonglow&#8217; and &#8216;Cameo&#8217; had 2n = 14. The F 1 hybrid from B. heterophylla &#8216;Red&#8217; &#215; B. megastigma had 2n = 15. Pollen viability, examined by using fluorescein diacetate, varied from 20% in B. crenulata to 80% in B. purdieana. Pollen of most species could be stored for 12 months in sealed containers at &#8722;20&#176;C. Drying with silica gel desiccant did not improve storage, nor did ultra-low temperature (&#8722;196&#176;C). Stored pollen was successfully used for interspecific hybridisation. 
ER  -

