
TY  - JOUR
A1  - Hall, Rhonda M. 
A1  - Drew, Roderick A. 
A1  - Higgins, Colleen M. 
A1  - Dietzgen, Ralf G. 
T1  - Efficient organogenesis of an Australian passionfruit hybrid (<emph type="2">Passiflora edulis</emph> x <emph type="2">Passiflora edulis</emph> var. <emph type="2">flavicarpa</emph>) suitable for gene delivery
JF  - Australian Journal of Botany
JO  - Aust. J. Bot.
Y1  - 2000
VL  - 48
IS  - 5
SP  - 673
EP  - 680
KW  - 
UR  - https://doi.org/10.1071/BT99067 
N2  - An efficient regeneration protocol based on organogenesis from cotyledon explants and suitable for gene delivery has been developed for an Australian passionfruit hybrid. Multiple shoots were regenerated from 30-day-old cotyledon explants on Murashige and Skoog (MS) medium containing 6-benzylvaminopurine (BAP) and coconut water. Media pulsing experiments were conducted to investigate the effect on organogenesis of exposure time of the explants to MS containing 10 &lowbar;M BAP and 10% (v/v) coconut water, i.e. passionfruit regeneration medium (PRM). Continuous exposure of these explants to PRM maximised the number of shoots produced to 12.1 per explant. However, periods on hormone-free medium improved the appearance of the shoots and increased the number of explants with shoots from 75 to 84.6%. Further, shoots exposed for 7 days to half-strength MS supplemented with 10 mM NAA (1-napthalene acetic acid) produced twice as many plantlets than those on half-strength MS alone. Transient GUS histochemical assays indicated delivery of the uidA gene via Agrobacterium tumefaciens. 
ER  -

