
%0 Journal Article
%A Turner, S. R. 
%A Touchell, D. H. 
%A Dixon, K. W. 
%A Tan, B. 
%T Cryopreservation of <emph type="2">Anigozanthos viridis</emph> ssp. <emph type="2">viridis</emph> and related taxa from the south-west of Western Australia
%J Australian Journal of Botany
%D 2000/12/08
%V 48
%N 6
%P 739-744
%K 
%R https://doi.org/10.1071/BT99070
%U https://www.publish.csiro.au/paper/BT99070 
%X Cryostorage of shoot apices of the perennial monocotyledonous species Anigozanthos viridis Endl. ssp. viridis (Haemodoraceae) was investigated by using a modified vitrification protocol. The highest post-thaw survival of shoot apices (41.4%) involved preculturing shoot apices on 0.4 M sorbitol for 48 h followed by incubation in a vitrification cryoprotective solution (PVS2) for 25 min at 0&#x02DA;C. The level and type of cytokinin used in the culture stage was also found to influence cryostorage success with post-thaw survival decreasing from 41% with zero to low levels of cytokinins to below 5% for cytokinin levels that are typical of plant tissue applications (2.5 &#732;M) for Australian plant species. Five Haemodoraceae taxa (Anigozanthos Labill. spp. and Conostylis R.Br. spp.) were successfully cryopreserved with this modified protocol; however, a sixth taxon, Macropidia fuliginosa (Hook.) Druce., remained unresponsive to this vitrification technique. 
