
TY  - JOUR
A1  - Godwin, ID 
A1  - Fordlloyd, BV 
A1  - Newbury, HJ 
T1  - <I>In vitro</I> Approaches to Extending the Host-Range of <I>Agrobacterium</I> for Plant Transformation
JF  - Australian Journal of Botany
JO  - Aust. J. Bot.
Y1  - 1992
VL  - 40
IS  - 6
SP  - 751
EP  - 763
KW  - 
UR  - https://doi.org/10.1071/BT9920751 
N2  - Since the first report of successful gene transfer in 1983, engineered Agrobacterium vectors have been the most widely used means of stable plant transformation. There are host-range limitations to the utility of this approach, however, with most success in dicotyledonous species. Recent evidence has indicated that the host-range is dependent on the protocol used to infect plant tissues with Agrobacterium. Modifications of co-cultivation conditions can lead to successful gene transfer to species once thought beyond the host-range of Agrobacterium, including a number of monocots. Hence, when defining the host-range of Agrobacterium, distinction must be made between host-range for pathogenicity (oncogenicity) and host-range for transformation. Important factors in determining the ability of Agrobacterium to transfer DNA to the plant genome include genetic, physiological and physical components of the bacterium &#215; plant interaction. Simple, empirical experiments with oncogenic strains can yield useful information regarding the importance of co-cultivation conditions. The applications of these to extending the host-range of Agrobacterium for transformation are discussed. 
ER  -

