
%0 Journal Article
%A Wagih, ME 
%A Gordon, GH 
%A Ryan, CC 
%A Adkins, SW 
%T Development of an Axillary Bud Culture Technique for Fiji Disease Virus Elimination in Sugarcane
%J Australian Journal of Botany
%D 1995/02/01
%V 43
%N 1
%P 135-143
%K 
%R https://doi.org/10.1071/BT9950135
%U https://www.publish.csiro.au/paper/BT9950135 
%X A technique was developed for culture of axillary buds of sugarcane (Saccharum spp. hybrid) cultivar NCo310. Successful establishment was obtained following surface sterilisation (sodium hypochlorite 4% for 5 min), decontamination (100 mg L-1 carbenicillin, 25 mg L-1 nystatin and 100 mg L-1 benlate) and a double heat treatment applied firstly to setts (single-bud vegetative cuttings; 50&#176;C for 120 min) and then to the isolated axillary buds (61&#176;C for 30 min). The most suitable bud culture medium consisted of half-strength Murashige and Skoog nutrients plus 8 g L-1 agar, 30 g L-1 sucrose, 2 mg L-1 naphthalene-acetic acid and 500 mg L-1 malt extract. Roots and hence plantlets could be developed for all cultures following incubation in distilled water. Decontamination and heat treatment resulted in eradication of pathogens from 94% and Fiji disease virus from 28% of the cultures. 
