
TY  - JOUR
A1  - Wagih, ME 
A1  - Gordon, GH 
A1  - Ryan, CC 
A1  - Adkins, SW 
T1  - Development of an Axillary Bud Culture Technique for Fiji Disease Virus Elimination in Sugarcane
JF  - Australian Journal of Botany
JO  - Aust. J. Bot.
Y1  - 1995
VL  - 43
IS  - 1
SP  - 135
EP  - 143
KW  - 
UR  - https://doi.org/10.1071/BT9950135 
N2  - A technique was developed for culture of axillary buds of sugarcane (Saccharum spp. hybrid) cultivar NCo310. Successful establishment was obtained following surface sterilisation (sodium hypochlorite 4% for 5 min), decontamination (100 mg L-1 carbenicillin, 25 mg L-1 nystatin and 100 mg L-1 benlate) and a double heat treatment applied firstly to setts (single-bud vegetative cuttings; 50&#176;C for 120 min) and then to the isolated axillary buds (61&#176;C for 30 min). The most suitable bud culture medium consisted of half-strength Murashige and Skoog nutrients plus 8 g L-1 agar, 30 g L-1 sucrose, 2 mg L-1 naphthalene-acetic acid and 500 mg L-1 malt extract. Roots and hence plantlets could be developed for all cultures following incubation in distilled water. Decontamination and heat treatment resulted in eradication of pathogens from 94% and Fiji disease virus from 28% of the cultures. 
ER  -

