
%0 Journal Article
%A Barton, Sarah J. 
%A Barnham, Kevin J. 
%A Habtemariam, Abraha 
%A Frey, Urban 
%A Sue, Rodney E. 
%A Sadler, Peter J. 
%T [<emph type="7">1</emph>H,<emph type="7">15</emph>N] N.M.R. Kinetic Studies of Reactions of <emph type="2">cis</emph>- and <emph type="2">trans</emph>-[PtCl<emph tye="8">2</emph>(<emph type="7">15</emph>NH<emph tye="8">3</emph>)(c-C<emph tye="8">6</emph>H<emph tye="8">11</emph><emph type="7">15</emph>NH<emph tye="8">2</emph>)] with Guanosine 5'-Monophosphate
%J Australian Journal of Chemistry
%D 1999/04/16
%V 52
%N 3
%P 173-178
%K 
%R https://doi.org/10.1071/C98168
%U https://www.publish.csiro.au/paper/C98168 
%X cis-&#91;PtCl2(15NH3)(c-C6H11NH2)&#93; is an active metabolite of the oral platinum(IV) anticancer drug cis,trans,cis-&#91;PtCl2(CH3CO2)2(NH2)(c-C6H11NH2)&#93;. Since it is likely that guanine bases on DNA are targets for this drug, we have analysed the kinetics of reaction of this platinum(II) metabolite with guanosine 5&#8242;-monophosphate (5&#8242;-GMP) at 310 K, pH 7, using &#91;1H, 15N&#93; n.m.r. methods. Reactions of the trans isomer are reported for comparison. The reactions proceed via aquated intermediates, and, for the cis isomer, the rates of aquation and substitution of H2O by 5&#8242;-GMP are 2-5 times faster trans to the amine ligand (c-C6H11NH2) compared to trans to NH3 for both the first and second steps. For the trans complex, the first aquation step is c. 3 times faster than for the cis complex, as expected from the higher trans influence of Cl&#175;, whereas the rate of the second aquation step (trans to N7 of 5&#8242;-GMP) is comparable to that trans to NH3. These findings have implications for the courses of reactions with DNA. 
