
TY  - JOUR
A1  - Holt, S. A. 
A1  - Henderson, M. J. 
A1  - White, J. W. 
T1  - Thermal Denaturation of Interfacial Protein Layers
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 2002
VL  - 55
IS  - 7
SP  - 449
EP  - 459
KW  - 
UR  - https://doi.org/10.1071/CH02100 
N2  - We report for the first time the structural measurements at nanometre resolution of the denaturation of &#946;-lactoglobulin and lysozyme at an air&#8211;water interface using the technique of neutron reflectivity. The incipient denaturation shown previously[1] for myogloblin is also studied for these molecules at room temperature, and denaturation is provoked by increasing the temperature of the solutions progressively to 75&#176;C. The change in the adsorbed protein layer thickness, its scattering length density and density distribution perpendicular to the surface as a function of increased temperature are reported and the data analysed in terms of a two-state model for the denaturation process. These measurements are relevant to an understanding of the way in which proteins at interfaces act as templates, for example, in biomineralization. 
ER  -

