
%0 Journal Article
%A Macdonald, James M. 
%A Stick, Robert V. 
%A Tilbrook, D. Matthew G. 
%A Withers, Stephen G. 
%T Synthesis with Glycosynthases: Cello-Oligomers of Isofagomine and a Tetrahydrooxazine as Cellulase Inhibitors
%J Australian Journal of Chemistry
%D 2003/02/03
%V 55
%N 12
%P 747-752
%K 
%R https://doi.org/10.1071/CH02165
%U https://www.publish.csiro.au/paper/CH02165 
%X Isofagomine and a carbohydrate-like tetrahydrooxazine, as their N-benzyloxycarbonyl derivatives, have been subjected to a glycosynthase in the presence of &#945;-D-glucopyranosyl fluoride as a glucosyl donor. In each case, after protecting group removal, a mixture of 1,4-&#946;-linked di-, tri-, and tetra-&#39;saccharides&#39; was obtained. These novel oligosaccharide derivatives were tested as inhibitors of the endo-glycanase Cex from Cellulomonasfimi. Affinities increased progressively as additional D-glucosyl residues were incorporated, which is consistent with the known substrate specificity of this enzyme. 
