
%0 Journal Article
%A Gee, Michelle L. 
%A Smith, Trevor A. 
%T Shedding Light on the Conformation of Proteins and Other Macromolecules at an Interface
%J Australian Journal of Chemistry
%D 2003/09/12
%V 56
%N 10
%P 1005-1012
%K 
%R https://doi.org/10.1071/CH03093
%U https://www.publish.csiro.au/paper/CH03093 
%X An attractive way to probe interfacial phenomena is through the utilization of total internal reflection-based spectroscopic methods, in which the evanescent field is used to photo-excite an absorbing species situated within the penetrating field. In this paper we discuss both absorbance and time-resolved fluorescence evanescent wave techniques that we have developed. We highlight how these techniques can be utilized successfully for interrogating the adsorption behaviour of proteins and polymers as a function of distance from a solid/solution interface into bulk solution. Information provided by these methods regarding how protein and polymer molecular conformation is affected by their adsorption/interaction at a surface is discussed with specific reference to poly(2-vinylpyridine), hen egg white lysozyme, and bovine serum albumin. 
