
TY  - JOUR
A1  - Gee, Michelle L. 
A1  - Smith, Trevor A. 
T1  - Shedding Light on the Conformation of Proteins and Other Macromolecules at an Interface
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 2003
VL  - 56
IS  - 10
SP  - 1005
EP  - 1012
KW  - 
UR  - https://doi.org/10.1071/CH03093 
N2  - An attractive way to probe interfacial phenomena is through the utilization of total internal reflection-based spectroscopic methods, in which the evanescent field is used to photo-excite an absorbing species situated within the penetrating field. In this paper we discuss both absorbance and time-resolved fluorescence evanescent wave techniques that we have developed. We highlight how these techniques can be utilized successfully for interrogating the adsorption behaviour of proteins and polymers as a function of distance from a solid/solution interface into bulk solution. Information provided by these methods regarding how protein and polymer molecular conformation is affected by their adsorption/interaction at a surface is discussed with specific reference to poly(2-vinylpyridine), hen egg white lysozyme, and bovine serum albumin. 
ER  -

