
%0 Journal Article
%A Martin, RF 
%T The chemistry of the DNA-p-Nitrophenylhydrazine reaction
%J Australian Journal of Chemistry
%D 1972/02/01
%V 25
%N 2
%P 401-406
%K 
%R https://doi.org/10.1071/CH9720401
%U https://www.publish.csiro.au/paper/CH9720401 
%X The chemistry of the reaction between DNA and pNPH has been investigated by isolation and analysis of the chromogen. The perchlorate of the cationic chromo-gen was prepared from the reineckate by anion exchange chromatography, and samples from both DNA-pNPH and furfuryl alcohol-pNPH reaction mixtures were found to have identical i.r. and p.m.r. spectra. The data obtained from the p.m.r. spectra were consistent with a reaction scheme involving formation of a pyridazinium cation which subsequently yields the chromophore on treatment with alkali. 
