
%0 Journal Article
%A Oconnor, J 
%A Aggett, A 
%A Williams, DR 
%A Stanley, RA 
%T <I>Candida cylindracea</I> Lipase-Catalyzed Hydrolysis of Methyl Palmitate in Detergentless Microemulsion and Paraffin/Water Biphasic Media
%J Australian Journal of Chemistry
%D 1991/01/01
%V 44
%N 1
%P 53-60
%K 
%R https://doi.org/10.1071/CH9910053
%U https://www.publish.csiro.au/paper/CH9910053 
%X The activity of lipase from Candida cylindracea has been investigated in the detergentless microemulsion media of n-hexane/propan-2-ol/water and in paraffin/water biphasic mixtures. Analysis of the product of hydrolysis, palmitic acid, was by a titrimetric method developed for non-specific assay of free fatty acids in these mixed-solvent media. The enzyme-catalysed hydrolysis of methyl palmitate was very slow in a complete non-aqueous system, but addition of small amounts of water led to an increased rate of reaction at atmospheric pressure and 37&#176;C. The ability of the enzyme to work in these mixed-solvent systems under very favourable experimental conditions makes it particularly amenable to practical and cost-effective transformations. 
