
TY  - JOUR
A1  - Oconnor, J 
A1  - Aggett, A 
A1  - Williams, DR 
A1  - Stanley, RA 
T1  - <I>Candida cylindracea</I> Lipase-Catalyzed Hydrolysis of Methyl Palmitate in Detergentless Microemulsion and Paraffin/Water Biphasic Media
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 1991
VL  - 44
IS  - 1
SP  - 53
EP  - 60
KW  - 
UR  - https://doi.org/10.1071/CH9910053 
N2  - The activity of lipase from Candida cylindracea has been investigated in the detergentless microemulsion media of n-hexane/propan-2-ol/water and in paraffin/water biphasic mixtures. Analysis of the product of hydrolysis, palmitic acid, was by a titrimetric method developed for non-specific assay of free fatty acids in these mixed-solvent media. The enzyme-catalysed hydrolysis of methyl palmitate was very slow in a complete non-aqueous system, but addition of small amounts of water led to an increased rate of reaction at atmospheric pressure and 37&#176;C. The ability of the enzyme to work in these mixed-solvent systems under very favourable experimental conditions makes it particularly amenable to practical and cost-effective transformations. 
ER  -

