
TY  - JOUR
A1  - Vrkic, Ana K. 
A1  - O'Hair, Richard A. J. 
A1  - Foote, Simon 
T1  - Fragmentation Reactions of All 64 Deprotonated Trinucleotide and 16 Mixed Base Tetranucleotide Anions via Tandem Mass Spectrometry in an Ion Trap.
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 2000
VL  - 53
IS  - 4
SP  - 307
EP  - 319
KW  - Deprotonated oligodeoxynucleotides; sequencing; tandem mass spectrometry.
UR  - https://doi.org/10.1071/CH99153 
N2  - The gas-phase fragmentation reactions of the &#91;M&#8211;H&#93;&#8211; ions derived from all 64 trinucleotides and 16 isomeric tetranucleotides each containing adenine, thymine, guanine and cytosine were examined using multistage mass spectrometry (MS/MS and MS3) collision-induced dissociation in an ion trap mass spectrometer. The initial fragmentation pathway involved neutral base loss predominantly from the 5&acute; terminus, along with minor 3&acute; and internal base loss in the order A &#62; T &#62; G &#62; C to form &#91;M&#8211;H&#8211;BH&#93;&#8211; &#8216;non-sequence&#8217; ions. The resultant &#91;M&#8211;H&#8211;BH&#93;&#8211; ions often fragment via intramolecular proton transfer with concomitant cleavage of the 3&acute; C&#8211;O phosphodiester bond to yield an ion&#8211;molecule complex, which undergoes either direct dissociation or proton transfer to yield the w-type or (a&#8211;B)-type &#8216;sequence&#8217; ions respectively. 
ER  -

