
TY  - JOUR
A1  - Bradford, AM 
A1  - Raftery, MJ 
A1  - Bowie, JH 
A1  - Tyler, MJ 
A1  - Wallace, JC 
A1  - Adams, GW 
A1  - Severini, C 
T1  - Novel Uperin Peptides From the Dorsal Glands of the Australian Floodplain Toadlet <I>Uperoleia inundata</I>
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 1996
VL  - 49
IS  - 4
SP  - 475
EP  - 484
KW  - 
UR  - https://doi.org/10.1071/CH9960475 
N2  - The dorsal glandular extract of the floodplain toadlet Uperoleia inundata contains more than 50 peptides: we report the amino acid sequences and bioactivity data of 13 of these. The peptides have been sequenced by using a combination of mass spectrometry and automated Edman sequencing. Ten of the peptides have been synthesized in order to confirm their structures, and to enable bioassays to be carried out. Ten peptides are host- defence agents, including ( i ) a powerful new neuropeptide of the tachykinin family which we name uperin 1.1 [ pGlu Ala Asp Pro Asn Ala Phe Tyr Gly  Leu Met (NH2)], and (ii) nine antibiotic peptides including five uperins 2 [e.g. uperin 2.1, Gly  Ile Val Asp Phe Ala Lys  Lys Val Val Gly Gly Ile Arg Asn Ala Leu Gly Ile (OH)], three uperins 3 [e.g. uperin 3.1, Gly Val Leu Asp Ala Phe  Arg  Lys Ile Ala Thr Val Val Lys Asn Val Val (NH2)] and uperin 4.1 [ Gly Val Gly Ser Phe Ile His Lys Val Val Ser Ala Ile  Lys Asn Val Ala (NH2)]. The function of the three other characterized peptides in the amphibian integument is not known, viz. ( i ) uperin 5.1 [ Phe  Gln  Phe Val Asn Pro Ser Asp Ile Val Phe  Gly Ser (OH)] and (ii) the two uperins 6 [e.g. uperin 6.1, Gly  Leu Ala Gly Ala Ile Ser Ser Ala Leu Asp Lys  Leu Lys  Gln Ser Gln  Leu  Ile Lys  Asn  Tyr Ala Lys  Lys  Leu  Gly  Tyr Pro Arg (OH)]. 
ER  -

