
%0 Journal Article
%A Raman, H. 
%A Platz, G. J. 
%A Chalmers, K. J. 
%A Raman, R. 
%A Read, B. J. 
%A Barr, A. R. 
%A Moody, D. B. 
%T Mapping of genomic regions associated with net form of netblotch resistance in barley
%J Australian Journal of Agricultural Research
%D 2003/12/15
%V 54
%N 12
%P 1359-1367
%K seedling resistance, Pyrenophora teres f. teres, QTL, validation.
%R https://doi.org/10.1071/AR03026
%U https://www.publish.csiro.au/paper/AR03026 
%X Quantitative trait loci (QTLs) associated with resistance to net blotch and their chromosomal locations were determined from analyses of doubled haploid progeny of Alexis/Sloop, Arapiles/Franklin, Sloop/Halcyon, and recombinant inbred lines of Sloop-sib/Alexis. Five QTLs on chromosomes 2H, 3H, and 4H were found to be associated with seedling resistance to the net form of net blotch. In Arapiles/Franklin and Alexis/Sloop populations, 4 significant QTLs explaining 9&#8211;17% of the variation in net blotch resistance were detected on 2H and 3H. A major locus, QRpts4L accounting for 64% of the variation in infection type, was detected on 4H in the Sloop/Halcyon population. In Sloop/Halcyon, 2 microsatellite markers, EBmac0906 and GMS089, and AFLP marker P13/M50-108, co-segregated and detected maximum variability for net blotch resistance as revealed by bootstrap analysis. EBmac0906 and Bmac0181 were validated in F2 progeny of an Ant29/Halcyon population and reliably predicted phenotypes of 93% of lines resistant and susceptible to net blotch. These markers may be used within breeding programs to select alleles favourable for net blotch resistance derived from Halcyon. 
