
%0 Journal Article
%A Gray, FV 
%A Weller, RA 
%A Pilgrim, AF 
%A Jones, GB 
%T The rates of production of volatile fatty acids in the rumen. III. Measurement of production <I>in vivo</I> by two isotope dilution procedures
%J Australian Journal of Agricultural Research
%D 1966/02/01
%V 17
%N 1
%P 69-80
%K 
%R https://doi.org/10.1071/AR9660069
%U https://www.publish.csiro.au/paper/AR9660069 
%X (1) Production of volatile acid in the rumen of sheep fed at 12-hourly intervals on a mixture of wheaten hay and lucerne hay has been measured by two isotope dilution procedures involving continuous infusion of 14C-labelled fatty acid into the rumen and determination of the concentration of 14&#176;C in the rumen acid throughout the feeding cycle. In the first procedure the varying rate of production was matched by a varying rate of infusion so that a constant concentration of label was maintained in the rumen acid. In the second procedure a constant rate of infusion was maintained and a mean value obtained for the concentration of label in the rumen acid during the cycle. Production values calculated from the data from both procedures were similar: approximately 5 moles of volatile acid were produced per kilogram of dry fodder. (2) This same rate of production was found for rations of 360, 450, and 675 g dry fodder fed at 12-hourly intervals. 
