
TY  - JOUR
A1  - Gray, FV 
A1  - Weller, RA 
A1  - Pilgrim, AF 
A1  - Jones, GB 
T1  - The rates of production of volatile fatty acids in the rumen. III. Measurement of production <I>in vivo</I> by two isotope dilution procedures
JF  - Australian Journal of Agricultural Research
JO  - Aust. J.  Agric. Res.
Y1  - 1966
VL  - 17
IS  - 1
SP  - 69
EP  - 80
KW  - 
UR  - https://doi.org/10.1071/AR9660069 
N2  - (1) Production of volatile acid in the rumen of sheep fed at 12-hourly intervals on a mixture of wheaten hay and lucerne hay has been measured by two isotope dilution procedures involving continuous infusion of 14C-labelled fatty acid into the rumen and determination of the concentration of 14&#176;C in the rumen acid throughout the feeding cycle. In the first procedure the varying rate of production was matched by a varying rate of infusion so that a constant concentration of label was maintained in the rumen acid. In the second procedure a constant rate of infusion was maintained and a mean value obtained for the concentration of label in the rumen acid during the cycle. Production values calculated from the data from both procedures were similar: approximately 5 moles of volatile acid were produced per kilogram of dry fodder. (2) This same rate of production was found for rations of 360, 450, and 675 g dry fodder fed at 12-hourly intervals. 
ER  -

