
TY  - JOUR
A1  - Hume, ID 
T1  - Synthesis of microbial protein in the rumen.  III.The effect of dietary protein
JF  - Australian Journal of Agricultural Research
JO  - Aust. J.  Agric. Res.
Y1  - 1970
VL  - 21
IS  - 2
SP  - 305
EP  - 314
KW  - 
UR  - https://doi.org/10.1071/AR9700305 
N2  - Protein production in the rumen of sheep fed on a virtually protein-free diet supplemented with urea and higher volatile fatty acids (VFA) and yielding 600 g digestible organic matter per day amounted to 90 g/day. When gelatin was substituted for the higher VFA and 50% of the urea nitrogen, microbial protein production remained at a similar level (91 g/day); with casein, production increased to 101 g/day, and with zein to 104 g/day. Nitrogen balances increased from 4.1 g/day in sheep fed on the casein diet to 5.5 g/day in those fed on the zein diet (P < 0.05). These values were both significantly higher than those for the urea/VFA or gelatin-containing diets, reflecting the different levels of microbial protein production on the respective diets. In addition, 44 g un-degraded zein left the rumen daily, accounting for the increase in nitrogen balance on this diet above that on the casein diet. Negligible amounts of nitrogen were recycled on the urea/VFA, gelatin, and casein treatments, but at least 7.5 g recycled nitrogen was utilized in the rumen daily on the zein diet. This is equivalent to 47 g protein, sufficient in itself to satisfy the maintenance requirement of the sheep for protein. The yields of protein from ruminal fermentation on the three protein treatments suggest that the maximum possible yield may exceed 20 g/100 g organic matter digested in the rumen. 
ER  -

