
TY  - JOUR
A1  - Batterham, TJ 
A1  - Shutt, DA 
A1  - Hart, NK 
A1  - Braden, AWH 
A1  - Tweeddale, HJ 
T1  - Metabolism of intraruminally administered [4-<sup>14</sup>C]formononetic and [4-<sup>14</sup>C]biochanin a in sheep
JF  - Australian Journal of Agricultural Research
JO  - Aust. J.  Agric. Res.
Y1  - 1971
VL  - 22
IS  - 1
SP  - 131
EP  - 138
KW  - 
UR  - https://doi.org/10.1071/AR9710131 
N2  - Two ovariectomized ewes were given a single intraruminal dose of [4-14C]- labelled isoflavone and slaughtered 6 hr later. In the ewe given [4-14C]biochanin A, labelled genistein was detected in addition to labelled biochanin A in the blood plasma, urine, kidney, liver, bile, contents of the rumen, and those of the small intestine. p-Ethylphenol, which is almost certainly a metabolite of biochanin A, was present in the urine but not labelled, which indicates that it originates from ring B of the isoflavone molecule. In the ewe given [4-14C]formononetin, a similar distribution of the isoflavone and its demethylation product, daidzein, was found. In addition, labelled equol was found in the blood plasma and urine. Another labelled metabolite, identified as O-desmethyl angolensin (2,4-dihydroxyphenyl p-hydroxy-ß-methylbenzyl ketone), was also found in the urine. In the urine from both ewes, there were also labelled light petroleuminsoluble acidic compounds but they were not identified. 
ER  -

