
%0 Journal Article
%A Forlani, Giuseppe 
%T Differential expression of 5-<emph type="2">enol</emph>-pyruvyl-shikimate-3-phosphate synthase isoforms in elicitor-treated, cultured maize cells
%J Functional Plant Biology
%D 2002/12/16
%V 29
%N 12
%P 1483-1490
%K Keywords: differential expression of isozymes, elicitors, EPSP synthase, Fusarium acuminatum, maize (Zea mays L.), oxidative burst, phytoalexin production.
%R https://doi.org/10.1071/FP02136
%U https://www.publish.csiro.au/paper/FP02136 
%X The expression of two 5-enol-pyruvyl-shikimate-3-phosphate synthase (EC 2.5.1.19) isoforms was investigated in Zea mays L. suspension-cultured cells following exposure to a fungal elicitor. Activity levels of isozyme II specifically increased soon after treatment, in strict connection with induction of phenylalanine ammonia-lyase (PAL) and attainment of a new free-phenylalanine homeostasis at a higher concentration. However, a few days later, activity of the other enzyme form was also significantly enhanced, concomitant with a sharp rise in overall amino acid content, a further increase in PAL level and a resumption of cell lysis. Besides strengthening the hypothesis that an entire set of genes encoding for shikimate pathway enzymes (whose expression is specifically involved in plant dynamic defence) may exist, a general change in the levels of several amino acids seems to point towards a reprogramming of their metabolism in elicited cells. 
