
TY  - JOUR
A1  - Schünmann, Petra H.D. 
A1  - Surin, Brian 
A1  - Waterhouse, Peter M. 
T1  - A suite of novel promoters and terminators for plant biotechnology. II. The pPLEX series for use in monocots
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2003
VL  - 30
IS  - 4
SP  - 453
EP  - 460
KW  - actin, gene expression vectors, intron, nanovirus, subterranean clover stunt virus (SCSV), ubiquitin.
UR  - https://doi.org/10.1071/FP02167 
N2  - A suite of plant expression vectors (pPLEX), constructed from the gene regulation signals from subterranean clover stunt virus (SCSV) genome, has previously been used in dicot transformation for a variety of applications in plant biotechnology. To assess their use for the transformation of monocots, a number of modifications were made to the basic vector series and assessed in rice. In their unmodified forms, the SCSV promoters directed low levels of gene expression, however, insertion of an intron between the promoter and the transgene open reading frame (analogous to the rice actin and maize ubiquitin promoter systems) increased transgene expression 50-fold. The expression patterns from the intron-modified SCSV (segments 4 and 7) promoters were very similar to those directed by the actin or ubiquitin promoters. All promoter systems investigated directed expression that appeared to be constitutive within leaf tissue, and localised to the epidermal and vascular tissues of the root. The pPLEX vectors described here are an important counterpart to the dicot pPLEX series and have the potential to be useful in monocot research and biotechnology. 
ER  -

