
%0 Journal Article
%A Ko, Kenton 
%A Banerjee, Susitna 
%A Innes, Jennette 
%A Taylor, Darcie 
%A Ko, Zdenka 
%T The Tic40 translocon components exhibit preferential interactions with different forms of the Oee1 plastid protein precursor
%J Functional Plant Biology
%D 2004/04/15
%V 31
%N 3
%P 285-294
%K plastid translocon, precursor protein interactions, targeting domains, transport.
%R https://doi.org/10.1071/FP03195
%U https://www.publish.csiro.au/paper/FP03195 
%X The transport of proteins across the plastid envelope involves a host of proteinaceous components that attend to varying structural needs of the process. This study focuses on interactions between two select forms (designated Tic40 and Toc36) of the Tic40-related components and different structural versions of the Oee1 precursor to dissect the components&#39; mode of operation. Interaction profiling revealed several features pertaining to how Tic40-related components might work during the transport process. The main operational features revealed are: (1) Tic40 interacts preferentially with Oee1 precursors containing only the plastid-targeting domain, (2) Toc36 interacts preferentially with Oee1 precursors containing both plastid- and thylakoid lumen-targeting domains, (3) carboxyl truncations to either the entire Oee1 precursor or Toc36 affect interactions negatively, and (4) the general reduction of Tic40-related protein levels in transgenic plants appears to exert a greater negative impact on endogenous Oee1 levels than the other proteins assessed, a trend that corroborates the findings of the protein interaction experiments. 
