
%0 Journal Article
%A Xu, Muyun 
%A You, Jiangfeng 
%A Hou, Ningning 
%A Zhang, Hongmei 
%A Chen, Guang 
%A Yang, Zhenming 
%T <i>Corrigendum to</i>: Mitochondrial enzymes and citrate transporter contribute to the aluminium-induced citrate secretion from soybean (<i>Glycine max</i>) roots
%J Functional Plant Biology
%D 2010/04/30
%V 37
%N 5
%P 478-478
%K citrate synthase, mitochondrion, organic acids efflux, resistance, tricarboxylate carrier.
%R https://doi.org/10.1071/FP09223_CO
%U https://www.publish.csiro.au/paper/FP09223_CO 
%X The concentration of soluble aluminium (Al) in the soil solution increases at low pH and the prevalence of toxic Al3+ cations represent the main factor limiting plant growth on acid soils. Citrate secretion from roots is an important Al-tolerance mechanism in many species including soybean. We isolated mitochondria from the roots of an Al-resistant soybean (Glycine max L.) cv. Jiyu 70 to investigate the relationship between citrate metabolism and Al-induced citrate secretion. Spectrophotometric assays revealed that the activities of mitochondrial malate dehydrogenase and citrate synthase increased and aconitase decreased with increasing of Al concentration (0&#8211;50 &#181;M) and duration of Al treatment (30 &#181;M Al, 0.5&#8211;9 h). Al-induced citrate secretion was inhibited by the citrate synthase inhibitor suramin, and enhanced by the aconitase inhibitor fluorocitric acid. Mersalyl acid, an inhibitor of a citrate carrier located in mitochondria membrane, also suppressed Al-induced citrate secretion. Transcript level of the mitochondrial citrate synthase gene increased in soybean roots exposed to Al, whereas expression of aconitase showed no significant difference. Expression of Gm-AlCT, a gene showing homology to Al-activated citrate transporters was also induced after 4 h in Al treatment. The Al-dependent changes in activity and expression of these enzymes are consistent with them supporting the sustained release of citrate from soybean roots. 
