
%0 Journal Article
%A Tcherkez, Guillaume 
%A Guérard, Florence 
%A Gilard, Françoise 
%A Lamothe, Marlène 
%A Mauve, Caroline 
%A Gout, Elisabeth 
%A Bligny, Richard 
%T <i>Corrigendum to</i>: Metabolomic characterisation of the functional division of nitrogen metabolism in variegated leaves
%J Functional Plant Biology
%D 2014/02/11
%V 41
%N 3
%P 330-330
%K 
%R https://doi.org/10.1071/FP12189_CO
%U https://www.publish.csiro.au/paper/FP12189_CO 
%X Many horticultural and natural plant species have variegated leaves, that is, patchy leaves with green and non-green or white areas. Specific studies on the metabolism of variegated leaves are scarce and although white (non-green) areas have been assumed to play the role of a &#x2018;nitrogen store', there is no specific studies showing the analysis of nitrogenous metabolites and the dynamics of nitrogen assimilation. Here, we examined the metabolism of variegated leaves of Pelargonium &#xd7; hortorum. We show that white areas have a larger N : C ratio, more amino acids, with a clear accumulation of arginine. Metabolomic analyses revealed clear differences in the chemical composition, suggesting contrasted metabolic commitments such as an enhancement of alkaloid biosynthesis in white areas. Using isotopic labelling followed by nuclear magnetic resonance or liquid chromatography/mass spectrometry, we further showed that in addition to glutamine, tyrosine and tryptophan, N metabolism forms ornithine in green area and huge amounts of arginine in white areas. Fine isotopic measurements with isotope ratio mass spectrometry indicated that white and green areas exchange nitrogenous molecules but nitrogen export from green areas is quantitatively much more important. The biological significance of the metabolic exchange between leaf areas is briefly discussed. 
