
%0 Journal Article
%A Poupard, Pascal 
%A Brunel, Nicole 
%A Leduc, Nathalie 
%A Viémont, Jean-Daniel 
%A Strullu, Désiré-Georges 
%A Simoneau, Philippe 
%T Expression of a <emph type="2">Bet v 1</emph> homologue gene encoding a PR 10 protein in birch roots: induction by auxin and localization of the transcripts by <emph type="2">in situ</emph> hybridization
%J Functional Plant Biology
%D 2001/02/02
%V 28
%N 1
%P 57-63
%K 
%R https://doi.org/10.1071/PP00040
%U https://www.publish.csiro.au/paper/PP00040 
%X In birch roots (Betula pendula Roth), two members of the Bet v 1 gene family which encode PR 10 proteins have previously been characterized. One of these members, named Bet v 1-sc1, is significantly induced in response to biotic or abiotic factors. We have analysed the expression of Bet v 1-sc1 in birch roots treated either with 1 M indole-3-acetic acid (IAA) or 1 M kinetin using reverse transcription&#8211;polymerase chain reaction (RT&#8211;PCR), northern blotting and competitive PCR. High accumulation of the Bet v 1-sc1 transcripts was recorded only after auxin application, while kinetin had no effect. By in situ hybridization, we have investigated the localization of Bet v 1-sc1 mRNA in birch roots after induction of the gene by root treatment with 1 M IAA. Using root tip sections, we showed that Bet v 1-sc1 is significantly expressed in the apical meristem and the procambium. In sections taken in the zone producing lateral roots, the presence of Bet v 1-sc1 was found at sites of emerging secondary root primordia. This first report of localization of Bet v 1-sc1 expression suggests that this gene could be involved in the processes leading to lateral root initiation. 
