
%0 Journal Article
%A Helliwell, Chris A. 
%A Olive, Mark R. 
%A Forster, Leigh Gebbie Richard 
%A Peacock, W. James 
%A Dennis, Elizabeth S. 
%T Isolation of an <emph type="2">ent</emph>-kaurene oxidase cDNA from <emph type="2">Cucurbita maxima</emph>
%J Functional Plant Biology
%D 2000/12/04
%V 27
%N 12
%P 1141-1149
%K 
%R https://doi.org/10.1071/PP00079
%U https://www.publish.csiro.au/paper/PP00079 
%X Four distinct cDNAs isolated from developing Cucurbita maxima Duchesne seeds included CYP701A1, a member of the same subfamily of cytochrome P450 enzyme as the Arabidopsis ent-kaurene oxidase GA3 which catalyses an early step in gibberellin (GA) biosynthesis. We have shown by complementation of the Arabidopsis ga3-2 mutant that the CYP701A1 cDNA encodes an ent-kaurene oxidase activity. Another cDNA, CYP88A2, encodes a protein which is in the same subfamily as the maize Dwarf3 protein and is likely to encode another GA biosynthetic enzyme catalysing a reaction between ent-kaurenoic acid and GA12. 
